Journal: Cell reports

Article Title: CGRP signaling links tumor-associated pain to immune evasion in oral squamous cell carcinoma

doi: 10.1016/j.celrep.2026.116994

Figure Lengend Snippet: (A and B) Representative images of OSCC tumor serial sections stained with either anti-Tubb3 (top, green outline), anti-TRPV1 (middle, red outline), or anti-CGRP (bottom, blue outline) from a patient with (A) and without (B) CGRPα + nerve innervation. Positive stains are indicated by red (TRPV1) or blue (CGRP) arrows. Tumor margins are indicated by a gray dashed line. Image magnification: 6× (left) and 40× (right). Scale bar: 200 μm. (C) Quantification of the percentage of total TRPV1 and CGRP-IR nerve area relative to total Tubb3-IR nerve area across tumor tissue sections from 23 patients with HNSCC. Density is reported as a stacked bar graph. (D) Quantification of the percentage of total CD8 T cell density per square millimeter. (E) Representative images of OSCC tumor serial sections with either a large nerve bundle and low anti-CD8 or small nerve presence and high anti-CD8 immunoreactivity. Scale bar: 150 μm. (F and G) Simple linear regressions were run between patient-reported pain, percentage of total CGRP-IR nerve area relative to total Tubb3-IR, and CD8 + T cell density relative to tumor area. Pain was measured by the FACT-HN additional question 12, “I have pain in my mouth, throat or neck” (FACT-HN10). The response to this question is rated on a scale of 0 (not at all) to 4 (very much). Spearman correlation r coefficients are listed on each graph; p < 0.05. Patient demographics are located in .

Article Snippet: Slides were incubated overnight in PBS +/+ containing 1% bovine serum albumin and 0.1% Tween 20 and one of the following primary antibodies: Rabbit anti-CGRP (1:500, Cell Signaling Technologies), Rabbit anti-TRPV1 (1:500, Alomone Labs), Guinea pig anti-TRPV1 (1:500, Millipore).

Techniques: Staining

Journal: The FASEB Journal

Article Title: Unraveling Endocannabinoid Signaling Pathways in Cisplatin‐Induced Ototoxicity

doi: 10.1096/fj.202502888RR

Figure Lengend Snippet: Identification of the principal ECS elements in UB/OC1 cells. Western blots of eCB receptors (A) CB 1 R, (B) CB 2 R, (C) TRPV1, (D) PPARα, (E) PPARδ, and (F) PPARγ. Western blots of the AEA biosynthetic enzymes (G) NAPE‐PLD and (H) ABHD4, and of the AEA degradative enzymes (I) FAAH and (J) NAAA. Western blots of the primary 2‐AG metabolic enzymes (K) DAGLα, (L) DAGLβ, and (M) MAGL, and of the additional 2‐AG hydrolases (N) ABHD6 and (O) ABHD12. Each protein is shown alongside positive controls from mouse tissues with respective GAPDH housekeeping. The whole Western blot bands are reported in Figures . (P) Endogenous levels of AEA and 2‐AG and of (Q) eCB‐like compounds in UB/OC1 cells quantified by UHPLC–MS/MS. The data are presented as a box plot with whiskers ranging from min to max values. Each eCB is presented alongside its chemical structure. (R) Levels of eCBs and eCB‐like compounds in UB/OC1 cells are represented as a heatmap ( n = 4). 2‐AG, 2‐arachidonoylglycerol; AEA, N ‐arachidonoylethanolamine; ABHD4/6/12, α/β hydrolase domain‐containing protein; B, brain; CB 1 R, cannabinoid receptor 1; CB 2 R, cannabinoid receptor 2; DAGLα/β, diacylglycerol lipases α and β; DHEA, docosahexaenoylethanolamine; EPEA, N ‐epoxyeicosatetraenoylethanolamine; FAAH, fatty acid amide hydrolase; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; H, heart; L, liver; LEA, N ‐linoleoylethanolamine; MAGL, monoacylglycerol lipase; NAPE‐PLD, N ‐acyl‐phosphatidylethanolamines‐specific phospholipase D; NAAA, N ‐acylethanolamine acid amidase; OEA, N ‐oleoylethanolamine; PEA, N ‐palmitoylethanolamine; POEA, N ‐palmitoleoylethanolamine; PPARα/γ/δ, peroxisome proliferator‐activated nuclear receptors α, γ, δ; SEA, N ‐stearoylethanolamine; TRPV1, transient receptor potential vanilloid receptor 1.

Article Snippet: TRPV1 , OriGene TA336871 , Rabbit , 1:1000 , Nonfat dry milk 5% , Super Signal West Pico Plus.

Techniques: Western Blot, Tandem Mass Spectroscopy

Journal: The FASEB Journal

Article Title: Unraveling Endocannabinoid Signaling Pathways in Cisplatin‐Induced Ototoxicity

doi: 10.1096/fj.202502888RR

Figure Lengend Snippet: Analysis of eCB receptors in UB/OC1 cells upon cisplatin treatment. (A–F) Representative Western blot bands and densitometric quantification of CB 1 R (A), CB 2 R (B), TRPV1 (C), PPARα (D), PPARδ (E), and PPARγ (F) with respective GAPDH housekeeping in VEH and CIS‐treated cells. Data is presented as mean ± SEM. Statistical analysis was performed using a two‐tailed unpaired Student's t ‐test ( n = 3) (*** p ≤ 0.001 vs VEH). Whole Western blot bands are reported in Figures . (G) Schematic representation of the immunofluorescence staining method using anti‐CB 2 R antibody. (H–J) Representative confocal images of VEH‐treated cells immunostained with anti‐CB 2 R (red) and counterstained with Hoechst nuclear dye (blue); (K–M) Representative confocal images of CIS‐ treated cells immunostained with anti‐CB2R (red) and counterstained with Hoechst nuclear dye (blue). Scale bar: 20 μm. (N) Schematic representation of the CB 2 R selective probe SiR‐8 live‐cell staining. (O–Q) Representative confocal microscopy images of VEH‐treated cells stained with CB 2 R SiR‐8 probe (red) and counterstained with Hoechst nuclear dye (blue); (R–T) Representative confocal microscopy images of CIS‐treated cells stained with CB 2 R SiR‐8 probe (red) and counterstained with Hoechst nuclear dye (blue). Scale bar: 75 μm. White dotted boxes show zoomed‐in views of UB/OC1 cells, and the corresponding fluorescence intensity histograms are representative of the yellow circle‐marked areas. Live‐cell time‐lapse video is provided in the . CB 1 R, cannabinoid receptor 1; CB 2 R, cannabinoid receptor 2; CIS, cisplatin; eCBs, endocannabinoids; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; PPAR α, γ, δ, peroxisome proliferator‐activated nuclear receptors α, γ, δ; TRPV1, transient receptor potential vanilloid receptor 1; VEH, vehicle.

Article Snippet: TRPV1 , OriGene TA336871 , Rabbit , 1:1000 , Nonfat dry milk 5% , Super Signal West Pico Plus.

Techniques: Western Blot, Two Tailed Test, Immunofluorescence, Staining, Confocal Microscopy, Fluorescence

Journal: Frontiers in Medicine

Article Title: Radiofrequency ablation reduces esophageal hypersensitivity in refractory non-erosive reflux disease

doi: 10.3389/fmed.2026.1687564

Figure Lengend Snippet: Representative immunohistochemical images of TRPV1 expression in esophageal tissues across patient groups (IHC, ×400). (A) Preoperative specimen showing characteristic TRPV1 immunoreactivity distributed throughout the esophageal wall structure. (B) Postoperative specimen demonstrating significantly attenuated and more sparse TRPV1 staining compared to preoperative levels, indicating reduced esophageal hypersensitivity following radiofrequency ablation therapy.

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 1 h at room temperature, then incubated with primary antibodies against PAR2 (Abcam, ab180953, 1:1000 dilution) or TRPV1 (Cell Signaling Technology, #8240, 1:1000 dilution) on a shaking platform at 4 °C overnight .

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Frontiers in Medicine

Article Title: Radiofrequency ablation reduces esophageal hypersensitivity in refractory non-erosive reflux disease

doi: 10.3389/fmed.2026.1687564

Figure Lengend Snippet: Quantification of TRPV1-positive cells across study groups ( p < 0.05).

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 1 h at room temperature, then incubated with primary antibodies against PAR2 (Abcam, ab180953, 1:1000 dilution) or TRPV1 (Cell Signaling Technology, #8240, 1:1000 dilution) on a shaking platform at 4 °C overnight .

Techniques:

Journal: Frontiers in Medicine

Article Title: Radiofrequency ablation reduces esophageal hypersensitivity in refractory non-erosive reflux disease

doi: 10.3389/fmed.2026.1687564

Figure Lengend Snippet: Protein expression of TRPV1 in esophageal tissues across experimental groups.

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 1 h at room temperature, then incubated with primary antibodies against PAR2 (Abcam, ab180953, 1:1000 dilution) or TRPV1 (Cell Signaling Technology, #8240, 1:1000 dilution) on a shaking platform at 4 °C overnight .

Techniques: Expressing

Journal: Frontiers in Medicine

Article Title: Radiofrequency ablation reduces esophageal hypersensitivity in refractory non-erosive reflux disease

doi: 10.3389/fmed.2026.1687564

Figure Lengend Snippet: Expression of TRPV1 in serum samples ( p < 0.05).

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST for 1 h at room temperature, then incubated with primary antibodies against PAR2 (Abcam, ab180953, 1:1000 dilution) or TRPV1 (Cell Signaling Technology, #8240, 1:1000 dilution) on a shaking platform at 4 °C overnight .

Techniques: Expressing

Journal: Frontiers in Medicine

Article Title: Radiofrequency ablation reduces esophageal hypersensitivity in refractory non-erosive reflux disease

doi: 10.3389/fmed.2026.1687564

Figure Lengend Snippet: Representative immunohistochemical images of TRPV1 expression in esophageal tissues across patient groups (IHC, ×400). (A) Preoperative specimen showing characteristic TRPV1 immunoreactivity distributed throughout the esophageal wall structure. (B) Postoperative specimen demonstrating significantly attenuated and more sparse TRPV1 staining compared to preoperative levels, indicating reduced esophageal hypersensitivity following radiofrequency ablation therapy.

Article Snippet: The sections were blocked with 3% bovine serum albumin (BSA) at room temperature for 30 min. After removing the blocking solution, Sections were incubated overnight at 4 °C with rabbit anti-human PAR2 polyclonal antibody (Abcam, ab180953, 1:200 dilution) or rabbit anti-human TRPV1 monoclonal antibody (Cell Signaling Technology, #8240, 1:100 dilution), and the sections were incubated overnight at 4 °C in a humidified chamber.

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Frontiers in Medicine

Article Title: Radiofrequency ablation reduces esophageal hypersensitivity in refractory non-erosive reflux disease

doi: 10.3389/fmed.2026.1687564

Figure Lengend Snippet: Quantification of TRPV1-positive cells across study groups ( p < 0.05).

Article Snippet: The sections were blocked with 3% bovine serum albumin (BSA) at room temperature for 30 min. After removing the blocking solution, Sections were incubated overnight at 4 °C with rabbit anti-human PAR2 polyclonal antibody (Abcam, ab180953, 1:200 dilution) or rabbit anti-human TRPV1 monoclonal antibody (Cell Signaling Technology, #8240, 1:100 dilution), and the sections were incubated overnight at 4 °C in a humidified chamber.

Techniques:

Journal: Frontiers in Medicine

Article Title: Radiofrequency ablation reduces esophageal hypersensitivity in refractory non-erosive reflux disease

doi: 10.3389/fmed.2026.1687564

Figure Lengend Snippet: Protein expression of TRPV1 in esophageal tissues across experimental groups.

Article Snippet: The sections were blocked with 3% bovine serum albumin (BSA) at room temperature for 30 min. After removing the blocking solution, Sections were incubated overnight at 4 °C with rabbit anti-human PAR2 polyclonal antibody (Abcam, ab180953, 1:200 dilution) or rabbit anti-human TRPV1 monoclonal antibody (Cell Signaling Technology, #8240, 1:100 dilution), and the sections were incubated overnight at 4 °C in a humidified chamber.

Techniques: Expressing

Journal: Frontiers in Medicine

Article Title: Radiofrequency ablation reduces esophageal hypersensitivity in refractory non-erosive reflux disease

doi: 10.3389/fmed.2026.1687564

Figure Lengend Snippet: Expression of TRPV1 in serum samples ( p < 0.05).

Article Snippet: The sections were blocked with 3% bovine serum albumin (BSA) at room temperature for 30 min. After removing the blocking solution, Sections were incubated overnight at 4 °C with rabbit anti-human PAR2 polyclonal antibody (Abcam, ab180953, 1:200 dilution) or rabbit anti-human TRPV1 monoclonal antibody (Cell Signaling Technology, #8240, 1:100 dilution), and the sections were incubated overnight at 4 °C in a humidified chamber.

Techniques: Expressing